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dimeric c met protein  (R&D Systems Hematology)

 
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    R&D Systems Hematology dimeric c met protein
    Dimeric C Met Protein, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dimeric+c+met+protein/Recombinant+Human+HGFR%2Fc-MET+Fc+Chimera+His-tag+Protein/us08673302-500-6-9
    Average 93 stars, based on 19 article reviews
    dimeric c met protein - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Anti-cMet antibody and its use for the detection and the diagnosis of cancer
    Article Snippet: .. To perform these ELISA the human dimeric c-Met protein (R&D sytems, cat #358MT) is coated at the concentration of 0.25 μg/ml in PBS overnight at 4° C. After saturation of the plates (Costar #3690) with a 0.5% gelatin solution 2 hours at 37° C., hybridoma supernatants are incubated 1 hour at 37° C. Once rinsed with PBS, the anti-mouse HRP-antibody (Jackson ImmunoResearch, catalog #115-035-164) is added to each well at a 1/5000 dilution in ELISA buffer (0.1% gelatin/0.05% Tween 20 in PBS) and the plates incubated for 1 hour at 37° C. After 3 washes in PBS, the activity of the peroxydase is revealed by the addition of 50 μl of TMB substrate (Uptima). ..

    Concentration Assay:

    Article Title: Anti-cMet antibody and its use for the detection and the diagnosis of cancer
    Article Snippet: .. To perform these ELISA the human dimeric c-Met protein (R&D sytems, cat #358MT) is coated at the concentration of 0.25 μg/ml in PBS overnight at 4° C. After saturation of the plates (Costar #3690) with a 0.5% gelatin solution 2 hours at 37° C., hybridoma supernatants are incubated 1 hour at 37° C. Once rinsed with PBS, the anti-mouse HRP-antibody (Jackson ImmunoResearch, catalog #115-035-164) is added to each well at a 1/5000 dilution in ELISA buffer (0.1% gelatin/0.05% Tween 20 in PBS) and the plates incubated for 1 hour at 37° C. After 3 washes in PBS, the activity of the peroxydase is revealed by the addition of 50 μl of TMB substrate (Uptima). ..

    Incubation:

    Article Title: Anti-cMet antibody and its use for the detection and the diagnosis of cancer
    Article Snippet: .. To perform these ELISA the human dimeric c-Met protein (R&D sytems, cat #358MT) is coated at the concentration of 0.25 μg/ml in PBS overnight at 4° C. After saturation of the plates (Costar #3690) with a 0.5% gelatin solution 2 hours at 37° C., hybridoma supernatants are incubated 1 hour at 37° C. Once rinsed with PBS, the anti-mouse HRP-antibody (Jackson ImmunoResearch, catalog #115-035-164) is added to each well at a 1/5000 dilution in ELISA buffer (0.1% gelatin/0.05% Tween 20 in PBS) and the plates incubated for 1 hour at 37° C. After 3 washes in PBS, the activity of the peroxydase is revealed by the addition of 50 μl of TMB substrate (Uptima). ..

    Activity Assay:

    Article Title: Anti-cMet antibody and its use for the detection and the diagnosis of cancer
    Article Snippet: .. To perform these ELISA the human dimeric c-Met protein (R&D sytems, cat #358MT) is coated at the concentration of 0.25 μg/ml in PBS overnight at 4° C. After saturation of the plates (Costar #3690) with a 0.5% gelatin solution 2 hours at 37° C., hybridoma supernatants are incubated 1 hour at 37° C. Once rinsed with PBS, the anti-mouse HRP-antibody (Jackson ImmunoResearch, catalog #115-035-164) is added to each well at a 1/5000 dilution in ELISA buffer (0.1% gelatin/0.05% Tween 20 in PBS) and the plates incubated for 1 hour at 37° C. After 3 washes in PBS, the activity of the peroxydase is revealed by the addition of 50 μl of TMB substrate (Uptima). ..



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